树菌结节的多样性和组成受三叶草宿主选择和当地生长条件的影响
Bryden Fields1, Sara Moeskjaer2,3, William J Deakin1
1Department of Biology, University of York, York, UK.
Molecular ecology
|May 30, 2023
概括
白三叶草品种和基因型显著塑造了它们的根结结细菌 (Rhizobium leguminosarum symbiovar trifolii) 社区. 宿主选择和当地条件共同影响这种共生生物的多样性和组成.
科学领域:
- 植物科学 植物科学
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 宿主过形成了豆类-生菌共生,但它对单一植物物种内的共生生物多样性的影响不太清楚.
- 了解植物品种和基因型如何影响微生物群落对于优化共生关系至关重要.
研究的目的:
- 调查白叶三叶草 (Trifolium repens) 品种和基因型对根结核 Rhizobium leguminosarum symbiovar trifolii (Rlt) 群体的多样性和组成的影响.
- 确定不同三叶草基因型对宿主过的程度及其与共生效率的相关性.
主要方法:
- 清洁和共生基因 (rpoB,recA,nodA,nodD) 的高通量安普利康序列测定,以分析根结块中的Rlt基因型多样性.
- 实地和受控温室试验比较不同Trifolium repens交叉和品种的Rlt社区.
- 基于植物绿色色调的共生效率的量化.
主要成果:
- 在实地试验中,在三叶草交叉之间观察到RLT结节多样性的显著差异,当地环境条件也发挥了作用.
- 有控制的温室实验表明,不同的三叶草品种和基因型对显著不同的Rlt结节群体进行选择.
- 宿主过的强度与共生效率有正相关,这表明宿主选择和共生效率之间存在联系.
结论:
- 宿主基因型选择和当地的环境条件共同决定了白三叶草的RLT结节多样性和社区组成.
- 白三叶草的品种和基因型对它们的共生树生物种群施加了显著的选择性压力.
- 宿主过强度是影响豆类-生菌共生效率的关键因素.
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