神经素通过促进降解和削弱其对基质的亲和力来影响神经化样1的活性
Jingling Zhu1,2, Yu Li2, Chen Zhong3
1Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Acta biochimica et biophysica Sinica
|May 30, 2023
概括
神经素通过降低活性并促进神经化样1 (Neurl1) 的降解来抑制Notch信号传递. 这就澄清了神经素的存在.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 神经素对于神经发育和再生至关重要.
- 之前显示的neuritin通过神经化样式1 (Neurl1) 抑制了Notch信号传输.
- 神经素调节Neurl1的确切机制尚不清楚.
研究的目的:
- 阐明神经素通过Neurl1.1调节Notch信号的分子机制.
- 为了研究神经素如何影响Neurl1的E3结合酶活性和蛋白质稳定性.
主要方法:
- 确认神经素作为Neurl1.1的上游调节剂.
- 评估Neurl1的无处不在和Jagged1.1的内细胞分裂.
- 测量Notch细胞内域 (NICD) 和HES1表达.
- 通过26S蛋白酶体通路评估Neurl1-Jagged1亲和力和Neurl1降解.
主要成果:
- 神经胺抑制了Neurl1的活性,减少了Jagged1的无处不在和内细胞分裂.
- 减少Neurl1活动导致NICD和HES1表达的减少.
- 神经素削弱了Neurl1-Jagged1的结合,并促进了Neurl1的降解.
结论:
- 神经素通过抑制Neurl1活动并促进其降解来负面调节Notch信号.
- 这项研究阐明了神经在痕通路调节中的作用.
- 提供了关于神经蛋白在神经再生和可塑性方面的机制的见解.
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