在干细胞中磁纳米颗粒的生物矿化
Alexandre Fromain1, Aurore Van de Walle1, Guilhem Curé1
1Laboratoire Physico Chimie Curie, CNRS UMR168, Institut Curie, Sorbonne Université, PSL University, 75005 Paris, France. claire.wilhelm@cnrs.fr.
Nanoscale
|May 30, 2023
概括
哺乳动物细胞可以从非磁铁前体生物合成磁性纳米粒子. 这项研究表明,在长时间的铁化后,干细胞中的磁性生物矿物化,揭示了细胞内铁的转化和磁相形成.
科学领域:
- 生物矿物化 生物矿物化
- 细胞的新陈代谢
- 纳米粒子生物合成
背景情况:
- 铁对人体生理学至关重要,参与运输,储存和氧化还原过程.
- 磁铁的存在和形成,特别是在大脑中,仍然未被充分探索.
- 之前的研究表明,干细胞可以从降解的合成分子中合成磁性纳米粒子.
研究的目的:
- 为了在哺乳动物细胞中证明磁性生物矿化,只使用非磁性铁盐前体.
- 研究细胞内磁性纳米粒子生物合成所需的条件.
- 为了描述在这个过程中形成的铁物种和阶段.
主要方法:
- 用铁酸盐化小鼠和人类介质干细胞,长达36天.
- 优化铁度和培养时间.
- 测量细胞内铁含量和细胞磁信号.
- 在FeK边缘的X射线吸收光谱 (XAS) 用于电子结构和化学环境分析.
主要成果:
- 在连续14天的铁化后,观察到磁性纳米粒子生物合成.
- 生物合成与显著的细胞内铁积累相关.
- XAS揭示了Fe2+与铁盐的Fe3+转化,以及铁和磁相的形成.
结论:
- 介质细胞干细胞可以从非磁铁前体中生物矿化磁性纳米粒子.
- 长时间暴露于铁是诱导这种磁性生物矿物化的关键.
- 该过程涉及将铁转化为Fe3+并形成铁和磁铁相.
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