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通过液体染色学和纸张喷雾离子移动性质谱法对细菌的菌株级别歧视

Orobola E Olajide¹, Yuyan Yi², Jingyi Zheng²

¹Department of Chemistry and Biochemistry, Auburn University, 179 Chemistry Building, Auburn, Alabama 36849, United States.

Journal of the American Society for Mass Spectrometry

|May 30, 2023

概括

区分像大肠杆菌 (E. coli) 这样的细菌菌株对于公共卫生至关重要. 新的液体染色学,离子移动性和并联MS方法使用脂质生物标志物准确识别大肠杆菌菌株,帮助控制抗生素耐药性.

科学领域:

分析化学分析化学
微生物学微生物学
生物化学生化学

背景情况:

准确的细菌菌株鉴定对于有效的医疗治疗和打击抗生素耐药性至关重要.
区分像大肠杆菌 (E. coli) 这样的细菌菌株存在重大公共卫生挑战.

研究的目的:

评估液体色谱,离子流动性和双重质谱 (LC-IM-MS/MS) 的有效性,以区分大肠杆菌菌株.
开发一种快速的方法,即纸喷射离子移动性-并联质谱法 (PS-IM-MS/MS),用于细菌菌株的识别.

主要方法:

使用LC-IM-MS/MS与多变量统计 (主要成分分析和线性分辨分析) 进行大肠杆菌菌株歧视.
采用纸张喷射离子移动性并列质谱 (PS-IM-MS/MS) 进行更快的分析,并采用数据融合技术.

主要成果:

对于大肠杆菌菌株的歧视,LC-IM-MS/MS实现了高预测率 (96.1%负,100%正离子模式).
特别是在数据融合方面,PS-IM-MS/MS显示出有前途的分类率 (高达80.5%),识别脂质异构体和适配体作为生物标志物.
在菌株之间发现的生物化学差异得到了比较基因组分析的证实.

结论:

LC-IM-MS/MS和PS-IM-MS/MS是有效的多维技术,用于细菌菌株分化.
PS-IM-MS/MS提供了一种快速,选择性和敏感的方法,用于在各种样本类型中区分细菌菌株.
已识别的脂质生物标志物为大肠杆菌菌株之间的生物化学变异提供了洞察力.

关键词:

大肠杆菌菌株分化的区别.环境的离子化环境.直接进行CCS测量.离子移动性的离子移动性双联质谱质量测量技术

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