三种乳酸细菌菌株的基因组序列草案研究了B维生素生物合成
Lu Gao1,2, Gyu-Sung Cho2, Erik Brinks2
1Department of Nutritional, Food, and Consumer Sciences, University of Applied Sciences Fulda, Fulda, Germany.
Microbiology resource announcements
|May 30, 2023
概括
研究人员对三种乳酸细菌进行了测序,确定了B族维生素合成的基因. 利莫西拉克托巴西勒 (Limosilactobacillus reuteri) 92071具有完整的维生素B12生物合成基因集群.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 营养生物化学 营养生物化学
背景情况:
- 乳酸细菌 (LAB) 在食品发酵和人类健康中至关重要.
- 了解LAB的代谢潜力,包括维生素生物合成,对于其应用至关重要.
- 基因组测序草案提供了细菌菌株的遗传构成和功能能力的见解.
研究的目的:
- 为了确定三个特定的LAB菌株的基因组序列草案:Limosilactobacillus reuteri 92071,Lactiplantibacillus plantarum 92117-i3和Limosilactobacillus fermentum 92072. 这三种菌株的基因组序列草案.
- 在这些细菌基因组中识别参与维生素B生物合成的基因.
- 分析被测序菌株的基因组特征,如GC含量.
主要方法:
- 在选择的乳酸细菌菌株上进行了全基因组测序.
- 进行了生物信息分析,以组装基因组草案并识别基因集群.
- 特别注意的是与维生素B合成途径相关的基因.
主要成果:
- 成功获得了Limosilactobacillus reuteri 92071,Lactiplantibacillus plantarum 92117-i3和Limosilactobacillus fermentum 92072的基因组序列草案.这些基因组序列已经得到了成功.
- 发现Lactobacillus reuteri 92071具有完整的基因集群,这些基因集群对于维生素B12的生物合成至关重要.
- 确定Limosilactobacillus reuteri 92071的GC含量为38.52%的摩尔.
结论:
- 测序的乳酸细菌菌株含有与B族维生素生产相关的基因.
- 乳杆菌reuteri 92071表现出对合成维生素B12的完整遗传能力,突出了其作为来源的潜力.
- 这些基因组发现有助于了解LAB代谢及其潜在的生物技术应用.
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