发展调节剂BldB在Streptomyces venezuelae中的全球影响
Marieta M Avramova1, Clare E M Stevenson2, Govind Chandra1
1Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom.
Journal of bacteriology
|May 30, 2023
概括
在Streptomyces中,BldB调节器控制着空中形的形成. BldB功能的丧失会提高关键基因的调节,包括iosA,当过度表达时,它会模仿发表型. 这揭示了BldBB.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 菌细菌为繁殖形成空中菌,这个过程由"头" (bld) 基因调节.
- 虽然已知许多bld基因功能,但BldB的作用尚不清楚.
- 委内瑞拉的Streptomyces拥有bldB,whiJ和abaA的多个类型.
研究的目的:
- 阐明BldB在Streptomyces发展中的全球监管作用.
- 为了识别由BldB.B.通过转录调节的基因.
- 了解BldB影响空中形形成的机制.
主要方法:
- 转录组测序 (RNA-seq) 用于分析bldB删除突变的基因表达变化.
- 进行过度表达研究以评估特定上调基因的表型影响.
- 进行了生物化学测试,以调查转录因子的结合和激活.
主要成果:
- 删除bldB导致了abaA对应物和新型化抑制剂iosA的显著上调.
- 对iosA和abaA6的共同过度表达使bldB突变表型 (头的外观) 的表现复制.
- 转录因子WhiJ9被确定为bldB突变体中iosA激活的关键,与基因间区域的调节元素结合.
结论:
- BldB在抑制关键发育基因的表达方面发挥着至关重要的作用,包括iosA和abaA对应基因.
- BldB蛋白可能与WhiJ9及其类型相互作用,形成一个调节复合体.
- 这项研究为管理Streptomyces发育和空中形形成的复杂监管网络提供了重要的新见解.
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