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一种高度可重复的电活性生物膜的分层设计,用于标准化生物化学氧气需求传感器

Huijuan Su¹, Xuejun Yan¹, Qian Zhao¹

¹MOE Key Laboratory of Pollution Processes and Environmental Criteria/Tianjin Key Laboratory of Environmental Remediation and Pollution Control/College of Environmental Science and Engineering, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin 300350, China.

|May 30, 2023

概括

使用Geobacter anodireducens设计的新型电活性生物膜 (DEAB) 为微生物电化学传感器提供了稳定和可重复的性能. 这一进步使生物化学氧需求 (BOD) 在现实应用中能够进行精确的测量.

科学领域:

生物电化学系统生物电化学系统
微生物电化学传感器
环境监测环境监测环境监测

背景情况:

微生物电化学传感器 (MES) 显示出监测生物可用有机物的潜力.
自然适应的电活性生物膜 (EAB) 的性能变化阻碍了实际应用.
对EAB的标准化对于可靠的传感器性能至关重要.

研究的目的:

设计一种稳定且可重复的电活性生物膜 (EAB),以提高传感器性能.
调查结构化EAB对生化氧需求 (BOD) 测量精度的影响.
为生物电化学技术中EAB标准化提出战略.

主要方法:

Geobacter anodireducens和其他微生物的顺序生长,以创建一个设计的EAB (DEAB).
用浮游生物有机生物作为氧气生物清除剂进行预注射,以促进Geobacter殖民.
使用DEAB对各种样本进行BOD测量的传感器的构建和测试.

主要成果:

DEAB实现了可重现的高电流密度,比自然适应的EAB (NEAB) 高4倍.
与NEAB相比,DEAB在复制品中表现出明显较低的变化 (15倍).
带有DEAB的传感器证明了对乙酸盐,废水和无氧消化超的更短的测量时间和精确的BOD测量.

结论:

成功开发了一种使用顺序生长和特定微生物联盟的EAB标准化策略.
设计的EAB (DEAB) 显著提高了微生物电化学传感器的稳定性和可重复性.
这种方法对于BOD传感器和其他生物电化学技术的可靠应用至关重要.

关键词:

地质细菌的数量减少了.生物化学的氧气需求需要.生物电化学系统生物电化学系统生物膜分层分层是生物膜的分层.微生物电化学传感器

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