通过花生 (Arachis hypogaea L.) 中的表型重组BSA/BSR同时绘制与两个植物架构特征相关的位点
Xiaona Yu1, Yaoyao Li1, Xinyuan Cui1
1Dry Farming Technology Key Laboratory of Shandong Province/College of Agronomy, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, People's Republic of China.
概括
一种新的方法,表型重组BSA/BSR (PR-BSA/BSR),同时确定两个花生特征的基因组区域. 这种方法准确地绘制了横向枝角和分离种群中的花枝图案的位置.
科学领域:
- 植物遗传学和基因组学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 大量分离分析测序 (BSA-seq) 是一种常见的方法,用于识别与特定特征相关的基因组区域.
- 花生植物的结构受到横向枝角 (LBA) 和花枝图案 (FBP) 的影响,影响作物产量和效率.
- 同时分析多个特征可以加速遗传发现.
研究的目的:
- 开发和验证一种新的方法,表型重组BSA/BSR (PR-BSA/BSR),用于同时识别与分离人口中的两个不同特征相关的基因组区域.
- 在花生F6种群中绘制控制LBA和FBP的遗传位置图.
- 与传统的QTL映射相比,评估PR-BSA/BSR方法的准确性和可靠性.
主要方法:
- 开发了PR-BSA/BSR方法,这是BSA/BSR的修改 (大量分离RNA测序).
- 从直立/序列 (ES) 和扩散/交替 (SA) 杂交的同系线的交叉生成F6花生种群.
- 选择具有极端重组表型 (扩散/序列和直立/交替) 的个体,以创建两个批量.
- 对个体的转录组测序和使用 ΔSNP-index 策略进行分析,以检测 LBA 和 FBP 的位置.
- 使用基于标记的定量特征位置 (QTL) 映射来确认结果.
主要成果:
- 通过PR-BSA/BSR方法,成功地同时确定了与LBA和FBP相关的候选基因组区域.
- 一个LBA的位置被映射到染色体15上的6.82Mb区域.
- 一个FBP的位点被映射到染色体12上的2.16Mb区域.
- 通过经典的QTL映射验证了结果,证实了PR-BSA/BSR方法的准确性.
结论:
- PR-BSA/BSR方法提供了一种准确可靠的手段,可以同时识别分离种群中的多个特征的基因组区域.
- 这种方法提高了植物复杂特征遗传映射的效率.
- 鉴定到的位点为标记器辅助的选择和花生结构的遗传改进提供了有价值的目标.
相关概念视频
Dihybrid Crosses
75.2K
Overview
75.2K
Trihybrid Crosses
23.5K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.5K
Monohybrid Crosses
230.5K
Overview
230.5K
Light Acquisition
8.5K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.5K
Law of Segregation
66.2K
When crossing pea plants, Mendel noticed that one of the parental traits would sometimes disappear in the first generation of offspring, called the F1 generation, and could reappear in the next generation (F2). He concluded that one of the traits must be dominant over the other, thereby causing masking of one trait in the F1 generation. When he crossed the F1 plants, he found that 75% of the offspring in the F2 generation had the dominant phenotype, while 25% had the recessive phenotype.
66.2K
Law of Independent Assortment
55.9K
While Mendel’s Law of Segregation states that the two alleles for one gene are separated into different gametes, a different question of how different genes are inherited remains. For example, is the gene for tall plants inherited with the gene for green peas? Mendel asked this question by experimenting with a dihybrid cross; a cross in which both parents are homozygous for two distinct traits resulting in an F1 generation that are heterozygous for both traits.
55.9K


