对不同碳源补充剂在全程和短途化-脱过程中对抗生素耐药性基因的影响的比较洞察
Meilan Zhang1,2,3, Kaiyi Li3, Panliang Wang3
1The State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, People's Republic of China.
概括
在短途化-脱过程中,酸有效地降低了垃圾填埋场液中的抗生素耐药性基因 (ARG) 和整合子. 然而,在全程处理期间添加甲醇显著增加了ARG,突出显示酸是减少ARG的最佳碳来源.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 抗生素耐药性 抗生素耐药性
背景情况:
- 成熟的垃圾填埋场液对去除具有挑战,并且是抗生素耐药性基因 (ARG) 的重要储库.
- 在捷径化和脱化中使用的外部碳来源可能会影响携带ARG的细菌,但这需要调查.
研究的目的:
- 为了研究不同外部碳来源 (葡萄糖,酸盐,甲醇) 在整个运行过程中对ARG的影响和垃圾填埋场液中的快捷除过程.
- 确定最佳的碳来源,以减少垃圾填埋污水处理中的ARG.
主要方法:
- 在不同碳源补充剂 (葡萄糖,酸,甲醇) 下对ARG丰度的比较分析.
- 处理过程的评估:全程与快捷化-脱化.
- 相关性和冗余性分析以确定碳来源,ARG,整体和细菌群落之间的关系.
主要成果:
- 在捷径过程中补充酸盐显著减少了总ARG和整体.
- 在整个运行过程中添加甲醇大大增加了总的ARG和整体.
- 移动基因元素intl1与大多数检测到的ARGs的传播有很强的相关性.
- 鉴定出细菌体和动菌体是关键ARG和移动遗传元素的潜在宿主.
结论:
- 酸是减少ARG和整体的最佳碳来源,在垃圾填埋污水的捷径化-脱化过程中.
- 碳来源和处理过程的选择显著影响了垃圾填埋场水中的ARG丰度和传播.
- 了解这些相互作用对于制定有效的策略来缓解废水处理中ARG传播至关重要.
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