一个生物信息指南,以识别来自植物病原体的蛋白质效应器
Christopher Blackman1, Rajagopal Subramaniam2
1Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.
Methods in molecular biology (Clifton, N.J.)
|May 30, 2023
概括
植物病原菌在全球范围内造成重大作物损失. 本综述侧重于识别蛋白质效应因子的方法,这些蛋白质效应因子是真菌用来克服植物防御和感染作物的方法,有助于减轻农业损失.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 植物病原菌导致全球实质性作物损失,估计每年15%.
- 了解宿主-病原体相互作用是制定减轻农业损失的战略的关键.
- 病原体分泌效应分子来抑制植物免疫力并促进植民.
研究的目的:
- 审查和总结识别来自植物病原菌的蛋白质效应物的方法.
- 提供专注于蛋白质效应因子识别的资源,与对小RNA或二次代谢物的审查不同.
主要方法:
- 本综述综合了关于用于蛋白质效应因子识别的各种技术的现有文献.
- 讨论的方法包括发现分泌的真菌蛋白和验证它们作为效应者的作用.
主要成果:
- 效应物是多种多样的,包括蛋白质,小RNA和真菌毒素.
- 蛋白质效应因子对于调节植物代谢和免疫反应至关重要.
- 有各种实验和计算方法可用于识别这些蛋白质效应器.
结论:
- 有效地识别真菌蛋白效应物对于理解植物防御机制至关重要.
- 基于效应体知识的开发策略可以帮助减少由真菌病原体引起的作物损失.
- 本综述是对植物病原菌中蛋白质效应因子发现的当前方法的指南.
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