时间序列预测和检测血液感染中的潜在病原体,使用mcfDNA测序
Yinghao Cao1,2, Tingting Jiang3,4, Yanfeng Lin4
1Department of Clinical Laboratory Medicine, School of Medicine, South China University of Technology, Guangzhou, China.
Frontiers in cellular and infection microbiology
|May 30, 2023
概括
微生物无细胞DNA (mcfDNA-seq) 的下一代测序显示出对血液感染 (BSI) 的高诊断灵敏度,在临床症状出现之前检测病原体. 这一进步有望为早期BSI检测提供更广泛的临床应用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 微生物无细胞DNA (mcfDNA-seq) 的下一代测序是一种正在发展中的血液感染 (BSI) 诊断工具.
- 需要临床验证来支持mcfDNA-seq用于早期病原体检测的应用.
研究的目的:
- 评估mcfDNA-seq在重症监护室 (ICU) 的高风险BSI患者的诊断和预测能力.
- 为了确定mcfDNA-seq是否可以识别前面的阳性血培结果的病原体.
主要方法:
- 测序mcfDNA-seq对高风险的ICU参与者的血液样本进行了测序.
- 血液培养结果作为比较的黄金标准.
- 在BSI发作之前和包括在内的七天内,从13名参与者收集了111个血液样本.
主要成果:
- mcfDNA-seq显示诊断灵敏度为90%,总预测灵敏度为87.5%.
- 通过mcfDNA-seq确定了大量的致病细菌,用于诊断和预测.
- 每百万病因因子的读数在BSI发作前几天显示出日益增长的趋势.
结论:
- mcfDNA-seq在识别与BSI相关的病原体方面表现出高的诊断灵敏度.
- 这种方法有可能在临床表现之前检测病原体,从而扩大其临床实用性.
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