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在膝关节骨关节炎患者中,肠道微生物组的变化概况

Xi Wang^1,2, Yifan Wu¹, Yanli Liu¹

¹Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Frontiers in microbiology

|May 30, 2023

概括

这项研究揭示了膝关节骨关节炎 (OA) 患者的独特肠道微生物群概况,确定了与疾病相关的特定细菌变化. 这些发现表明,肠道微生物组轴在OA病变发生过程中起着至关重要的作用.

科学领域:

微生物组研究微生物组研究
胃肠病学胃肠病学
整形外科整形外科整形外科

背景情况:

骨关节炎 (OA) 是一种慢性退行性关节疾病,主要原因不明.
肠道微生物群在包括OA在内的全身性疾病中的作用是一个新兴的研究领域.
了解OA患者的肠道微生物变化对于阐明疾病机制至关重要.

研究的目的:

在患有膝关节骨关节炎 (OA) 的患者中描述肠道微生物群组成.
确定特定的细菌属和物种,在OA患者和健康对照人群之间,它们的丰富程度有所不同.
探索肠道微生物失调症和膝关节OA的病变发生之间的潜在联系.

主要方法:

从32名膝关节骨质炎患者和57名健康对照组 (NC) 收集了便样本.
使用16S rDNA基因测序来分析肠道微生物群的组成.
使用元基因组测序来识别功能基因和物种级微生物变化.

主要成果:

蛋白质细菌在OA组中占主导地位.
在81个属中观察到显著的差异;特别是在OA患者中,Agathobacter,Ruminococcus,Roseburia,Subdoligranulum和Lactobacillus的丰度下降.
在OA患者中发现了Prevotella_7,Clostridium,Flavonifractor,Klebsiella,Escherichia coli,Klebsiella pneumoniae,Shigella flexneri和Streptococcus salivarius的丰富性增加,而Bacteroides物种则减少. 在OA患者中发现了Prevotella_7,Clostridium,Flavonifractor,Klebsiella,Escherichia coli,Klebsiella pneumoniae,Shigella flexneri和Streptococcus salivarius的丰富性增加,而Bacteroides物种则减少.

结论:

这项研究为膝盖骨关节炎患者提供了全面的肠道微生物群概况.
这些发现支持了"软骨-肠道-微生物组轴"影响OA发展的假设.
肠道微生物失生症可能在骨关节炎的发病过程中发挥重要作用.

关键词:

16s rDNA 测序的测序方法软骨-肠道-微生物群轴肠道微生物群生物标志物肠道微生物群生物标志物大基因组测序的测序.骨关节炎是一种关节炎.

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