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Updated: Jul 28, 2025

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使用CRISPR-Cas12a基于特定序列标签检测Burkholderia pseudomallei的检测

Jia-Xin Zhang1, Jian-Hao Xu1, Bing Yuan1

  • 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

Frontiers in public health
|May 30, 2023
PubMed
概括

一种新的双重目标方法能够快速准确地检测出Burkholderia pseudomallei (B. pseudomallei),这是导致性疹的原因. 这种RPA-CRISPR/Cas12a测定有助于及时诊断和控制这一重大公共卫生威胁.

关键词:
伯克霍尔德里亚是假名的.这就是CRISPR-Cas12a.种类歧视 种类歧视特定的序列标签的标签.视觉检测 视觉检测 视觉检测

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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