拓关联域是长基因间非编码RNAs的组织特异表达的基础
Yu Hamba1,2, Takashi Kamatani1,3,4,5, Fuyuki Miya6
1Laboratory for Medical Science Mathematics, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan.
iScience
|May 30, 2023
概括
长跨基因非编码RNAs (lincRNAs) 比蛋白质编码基因更具组织特异性. 它们在拓关联域 (TADs) 内的位置影响了这种特异性,为基因调节提供了洞察力.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 与蛋白质编码基因 (PCG) 相比,长跨基因非编码RNA (lincRNAs) 呈现出明显的组织特异性表达.
- 尽管它们具有正规的转录调节,但依据lincRNA表达特异性的调节机制尚未完全理解.
研究的目的:
- 研究基因组结构,特别是拓关联域 (TADs) 与lincRNAs的组织特异表达之间的关系.
- 开发一个框架来解释使用lincRNAs作为指标的转录状态.
主要方法:
- 对人类组织表达数据和拓关联域 (TAD) 坐标的分析.
- 在TAD中对lincRNA和PCG位点分布的比较分析.
- 应用一种新的分析框架,对过度缩性心肌病的数据.
主要成果:
- 与PCG相比,lincRNA位点在内部TAD区域中具有显著的丰富性.
- 位于TADs内的lincRNAs表现出比TADs外的组织特异性更高的组织特异性.
- 特定于疾病的转录性变化被确定为高性心肌病,包括质蛋白外宫表达,E2F1介导的肌细胞分化基因减压和LINC00881下调.
结论:
- 基因组架构,特别是TAD组织,在特定组织的lincRNAs表达中起着至关重要的作用.
- lincRNAs可以作为转录状态和疾病特异性调节事件的有价值指标.
- 这项研究为基因组结构的背景下lincRNA功能和调节提供了新的见解.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
01:30General Transcription Factors
5.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.4K
01:20Types of RNA
5.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
5.9K
02:16Transcription Factors
76.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.2K
03:18Non-LTR Retrotransposons
11.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.6K
03:17Inheritance of Chromatin Structures
6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K