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单重离心法和双重离心方法的比较,用于制备富血小板血 (PRP)

Nazish Saqlain¹, Naghmana Mazher², Tooba Fateen³

¹Nazish Saqlain, FCPS Hematology, Associate Professor of Pathology. The Children's Hospital and UCHS, Lahore, Pakistan.

Pakistan journal of medical sciences

|May 30, 2023

概括

与单次离心法相比,双重离心法产生显著更高的血小板计数和富血小板血 (PRP) 中的度. 这种方法还减少了红细胞 (RBC) 和白细胞 (WBC) 的污染,使其优于PRP制备.

科学领域:

血液学血液学血液学
输血医学输血医学
生物技术是生物技术.

背景情况:

富血小板血 (PRP) 在再生医学中被广泛使用.
标准化的制备方案对于最佳的PRP疗效至关重要.
对比单重和双重离心法对于提高PRP质量至关重要.

研究的目的:

在PRP中比较血小板计数,度/产量以及残余红血细胞 (RBC) 和白血细胞 (WBC) 的水平.
评估PRP制备的单反或双反离心方案的有效性.

主要方法:

一项涉及50名健康志愿者 (20-45岁) 的横截面研究.
在三酸管中采集的血液样本.
使用单一 (I组) 和双重离心 (II组) 方法制备的PRP.
使用自动化血液学分析仪 (SYSMEX XP-100) 分析血小板,红细胞和白细胞计数.

主要成果:

双离心 (II组) 与单次离心 (I组) (594.6±157.4×10^3/μl) 相比,平均血小板计数 (923.06±127.58×10^3/μl) 显著更高 (p <0.01).与单次离心相比,双离心 (II组) 的平均血小板计数 (923.06±127.58×10^3/μl) 显著更高 (p <0.01).
血小板度/产量在II组 (276.78 ± 112.7%) 与I组 (175.75 ± 55.08%) 相比显著更高 (p < 0.01).
第二组PRP显示WBC数量明显较低 (p <0.01) 和红细胞水平与I组相比相当.

结论:

双重离心方案在制备PRP方面优越,导致更高的血小板数量和产量.
与单次离心法相比,这种方法可以减少红细胞和白细胞的污染.
双离心法对自主和异性PRP制剂都有好处.

关键词:

血液血液血液血液离心机离心机离心机的使用方法血小板度血小板度是什么富含血小板血的血含量是多少

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