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Updated: Aug 4, 2026

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对于菌体显示白蛋白结合的亲和常数的确定

Yi-Feng Shi1

  • 1Department of Biotechnology, School of Biological Engineering, Dalian Polytechnic University, Dalian, Liaoning Province, P. R. China.

PeerJ
|May 30, 2023
PubMed
概括

这项研究引入了一种定量方法,以改善对人类血清白蛋白 (HSA) 结合的菌体显示查. 这种新方法提高了效率,并降低了用于药物开发的有效HSA结合剂的识别成本.

关键词:
亲和力常数是一种亲和力常数.结合的结合.毒品运输商 毒品运输商人类血清专素的人类血清专素划分标准 划分标准体显示器是体显示器.菌体标位的标位

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Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...