动态和自我生物降解的多糖体水凝在环境条件下储存胚胎干细胞结构
Kuan Yang1, Wei Wei1, Li Ting Gao1
1College of Bioresources Chemical and Materials Engineering, National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science and Technology, Xi'an, China.
Frontiers in bioengineering and biotechnology
|May 30, 2023
概括
我们开发了一种新的动态水凝结构,用于存储和运输小鼠胚胎干细胞 (mESC). 这种方法确保了高的存活率,并保留了用于"现成"干细胞应用的多能性.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 组织工程是组织工程.
背景情况:
- 胚胎干细胞 (ESC) 需要特定的微环境来储存和运输.
- 目前的方法在运输期间保持细胞活力和功能方面面临挑战.
- 对于研究和治疗应用的容易获得,可行的干细胞的需求正在增加.
研究的目的:
- 开发一种在环境条件下存储和运输ESC的新方法.
- 创建一个"现成"的解决方案,用于使用动态水凝进行干细胞输送.
- 评估ESCs存储和运输在水凝结构中的可行性,多能性和长期培养能力.
主要方法:
- 鼠标ESCs (mESCs) 被封装在一个自我生物降解的,动态的,基于多糖的水凝中,以形成ESCs-动态水凝结构 (CDHC).
- CDHC在无菌,密封条件下储存了6天,包括运输模拟.
- 运输后,mESCs从水凝中释放并培养15代,以评估恢复和多能性.
主要成果:
- 大型,紧的mESC殖民地在储存和模拟运输后保持了90%的生存率和多能性.
- 封装的干细胞在抵达时自动从可自降解的水凝中释放出来.
- 获取的mESCs在15代次培养后在蛋白质和mRNA水平上显示恢复了殖民地形成能力和多能性标志物.
结论:
- 动态的,可自我生物降解的水凝为ESCs的环境存储和运输提供了一种简单,经济高效的方法.
- 这种方法促进了可行的干细胞的"现成"可用性.
- CDHC技术支持干细胞在研究和潜在的临床环境中的广泛应用.
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