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单细胞分辨率的性别偏差基因表达:性二态的原因和后果

Iulia Darolti^1,2, Judith E Mank¹

¹Department of Zoology and Biodiversity Research Centre, University of British Columbia, Vancouver, BC, Canada.

Evolution letters

|May 30, 2023

概括

单细胞RNA测序揭示了细胞类型的差异,而不仅仅是基因调节,驱动了古皮的性别偏差基因表达. 这一发现对于理解性二态演化至关重要.

科学领域:

进化生物学是进化的生物学.
基因组学就是基因组学.
发育生物学是发展生物学.

背景情况:

性偏差基因表达与性二态进化有关.
大量基因表达研究可以将调控变化与细胞类型丰度的发育差异混为一谈.

研究的目的:

区分性别偏差基因表达的调节性与发育性原因.
研究细胞异质性对推断的性别偏差基因的影响.

主要方法:

从雄性和雌性古皮的体和生殖组织的单细胞RNA测序.
在单细胞分辨率下对基因表达的分析.
评估细胞类型的丰度和全米缩放.

主要成果:

细胞异质性和细胞类型丰富性的发育差异显著影响感知到的性别偏差基因表达.
这些因素可以增加批量分析中的假阳性和假阴性率.
细胞类型丰富性的性别差异可以混编码序列进化模式.

结论:

单细胞分辨率对于准确识别调控性偏差基因至关重要.
细胞类型丰富性的性别差异是性变态的后果,而不是原因.
了解细胞组成是研究性别特异性选择的关键.

关键词:

所有度测法 (allometry) 是一种全度测法.不同的表达方式,不同的表达方式.监管演变的监管演变通过scRNA测序进行测序.组织成分组织成分

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