在固态发酵下模拟和在生物体中评估由细菌细菌 (Bacillus subtilis) 生产的纤维解酶
Maysa E Moharam1, Magda A El-Bendary1, Mostafa M Abo Elsoud2
1Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Egypt.
Heliyon
|May 30, 2023
概括
细菌微Egy产生一个纤维解酶用于治疗血栓. 优化的固态发酵产生了高酶活性,在体内没有显著的毒性,显示了治疗潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 血液凝块导致心肌梗塞和中风,需要新型血栓解毒剂.
- 微生物纤维解酶为治疗血栓事件提供了一个有前途的途径.
研究的目的:
- 为了优化细菌细菌Egy使用固态发酵纤维解酶的生产.
- 评估产生的酶的体外和体外安全性和有效性.
主要方法:
- 使用各种营养来源的Bacillus subtilis Egy 固态发酵.
- 统计建模以优化发酵条件 (料酵母,水分,化,注射剂).
- 在体外和体外细胞毒性测定,包括大鼠的血液学和组织病理学检查.
主要成果:
- 作为营养来源的料酵母产生了最高的初始酶活性 (114 U/g).
- 优化条件 (3.6%的料酵母,40%的水分,6天,2%的注射剂) 将产量最大化至141.02U/g.
- 该酶在体内没有显示出死亡率或对血液学参数或器官组织学显著不良影响.
结论:
- 优化的固态发酵有效地增强了Bacillus subtilis Egy. 的纤维解酶产量.
- 产生的酶安全用于治疗,没有显著的细胞毒性或生理学破坏.
- 这种微生物酶具有作为治疗血栓的安全和有效的血栓抑制剂的潜力.
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