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鉴定与前列腺癌瘤发生相关的枢纽基因

Honghui Zhu¹, Qi Lin¹, Xiaomin Gao¹

¹Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Frontiers in oncology

|May 30, 2023

概括

包括SPP1,MYLK和ACTA2在内的7个枢纽基因被确定为前列腺癌 (PCa) 发展的关键参与者. 它们的异常表达驱动PCa细胞生长和迁移,为这种常见的恶性瘤提供潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

前列腺癌 (PCa) 是一种普遍存在的恶性瘤,其潜在机制尚不清楚.
了解PCa的分子驱动因素对于开发有效治疗方法至关重要.

研究的目的:

识别潜在的枢纽基因,阐明前列腺癌中涉及的机制.
探索PCa的新生物标志物和治疗点.

主要方法:

从基因表达综合 (GEO) 数据库中整合了两个队列基因表达特征数据集 (GSE55945,GSE6919).
利用生物信息学工具 (DAVID,STRING,Cytoscape,GEPIA,OmicStudio) 进行差异基因表达分析,通路丰富,蛋白质-蛋白质相互作用网络构建和预后分析.
使用定量逆转录PCR和西部抹杀验证的枢纽基因表达.

主要成果:

在PCa中确定了134个差异表达基因 (DEGs),其中14个是上调的,120个是下调的.
丰富分析揭示了DEGs参与细胞粘附,细胞外基质,迁移和血管光滑肌肉收缩.
确定了七个关键的枢纽基因 (SPP1,MYLK,MYL9,MYH11,CALD1,ACTA2,CNN1) 与PCa有显著的关联,通过验证研究证实了异常表达模式.

结论:

已确定的枢纽基因 (SPP1,MYLK,MYL9,MYH11,CALD1,ACTA2,CNN1) 与前列腺癌的发生有显著的相关性.
这些基因的异常表达促进PCa细胞的形成,增殖,入侵,迁移和瘤新血管化.
这些枢纽基因代表了前列腺癌患者潜在的诊断生物标志物和治疗点.

关键词:

生物信息学分析分析生物标志物生物标志物不同表达的基因 (DEGs)核心基因核心基因核心基因前列腺癌是前列腺癌.

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