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在RiPP生物合成期间的宏循环和脊柱重组,由SAM依赖的未知功能的域692进行生物合成

Richard S Ayikpoe^1,2, Lingyang Zhu³, Jeff Y Chen^1,2

¹Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, 61801, Illinois, United States.

ACS central science

|May 30, 2023

概括

研究人员确定了ChrH,这是DUF692家族中的一种新型酶,它催化了一种独特的化学转化. 这一发现扩大了这些含铁酶的已知功能,并提出了一个新的家族名称:多核非血铁依赖氧化酶 (MNIOs).

科学领域:

生物化学生物化学
酶学是一种酶学.
自然产品化学化学

背景情况:

DUF692 (功能域不明692) 家族包括新兴的翻译后修饰酶.
这些酶在合成核糖体合成和翻译后改性 (RiPP) 的天然产品中至关重要.
DUF692酶是含铁的多核酶,以前只有MbnB和TglH的特征.

研究的目的:

为了研究DUF692家族的另一个成员,ChrH,在*Chryseobacterium*物种中发现.
为了阐明由ChrH-ChrI酶复合物催化的化学转化.
提出一个反应机制,并重新分类DUF692家族.

主要方法:

生物信息分析以确定ChrH及其伴侣蛋白ChrI.
ChrH反应产物的结构特征.
同位素标记研究以确定反应机制.

主要成果:

ChrH-ChrI复合物催化了一种前所未有的反应,形成一个宏循环,imidazolidinedione异循环,thioaminals和一个 thiomethyl 组.
确定了第一个由DUF692酶复合物催化的S-adenosylmethionine (SAM) 依赖反应.
一个为基质的四电子氧化和甲基化提出的机制.

结论:

ChrH扩展了已知的DUF692酶的化学谱.
这项研究确定了一种新的SAM-依赖的酶反应.
基于特征成员的建议,重新命名DUF692家族为多核非血红素铁依赖氧化酶 (MNIOs).

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