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Updated: Jul 28, 2025

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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基酸激活在真菌非核糖体合成中,通过局部定向突变性评估

Sylvester Hoffmann1, Maik Damm1, Leonard Roth1

  • 1Fachgebiet Biologische Chemie, Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 124, 10623, Berlin, Germany.

Chembiochem : a European journal of chemical biology
|May 30, 2023
PubMed
概括

这项研究揭示了真菌的环二 (CDPs) 如何使用同质模型和突变分析激活酸. 非核糖体合成酶 (NRPS) 中的酸选择涉及骨干相互作用,而不是特定的侧链.

关键词:
在此之前,NRPS是NRPS.基化域的基化域.生物合成生物合成菌的循环代谢类类.突变发生的突变发生.自然产品是自然产品的产品.

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相关概念视频

Amides to Carboxylic Acids: Hydrolysis01:28

Amides to Carboxylic Acids: Hydrolysis

Amides can undergo either acid-catalyzed hydrolysis or base-promoted hydrolysis through a typical nucleophilic acyl substitution. Each hydrolysis requires severe conditions.
Acid-catalyzed hydrolysis:
Hydrolysis of amides under acidic conditions yields carboxylic acids. Since the reaction occurs slowly, hydrolysis requires the conditions of heat.
The mechanism begins with the protonation of the carbonyl oxygen by the acid catalyst. The protonation makes the amide carbonyl carbon more...
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