在野生Trametes versicolor菌株中, (III) 诱导的氧化防御和物种变化
Yuhui Zhang1, Xiaohong Chen1,2,3, Chenyu Wang1
1Horticulture College, Hunan Agricultural University, Changsha, Hunan, P. R. China.
PloS one
|May 30, 2023
概括
特拉梅特斯Versicolor通过增强抗氧化活性,特别是谷氨,有效地耐受高 (As) 水平. 这种真菌将有毒的As III转化为不那么有害的As V,使其成为污染环境中一个有希望的生物积累器.
科学领域:
- 环境微生物学环境微生物学
- 生物修复是一种生物修复.
- 菌类学 菌类学是指菌类学.
背景情况:
- (As) 污染对环境和健康构成重大风险.
- 在应激下,像Trametes versicolor这样的真菌的排毒机制尚未完全理解.
- 研究真菌对毒性的反应对于开发生物修复策略至关重要.
研究的目的:
- 为了阐明氧化防御机制和物种变化的变化在压力下的Trametes versicolor.
- 评估T. versicolor作为污染环境中的生物蓄积器的潜力.
主要方法:
- 在不同度的III (As III) 压力 (40和80毫克/升) 下种植野生T. versicolor HN01菌株.
- 使用微板阅读器量化抗氧化活性 (甲酶,超氧化物脱酶,谷氨).
- 使用高性能液态染色学与诱导合等离子体质谱法 (HPLC-ICP-MS) 结合确定真菌菌中的物种化.
主要成果:
- T. versicolor HN01对As III具有显著的耐受性,能够承受高达80mg/L的度,并表现出11.25.25的生物丰富系数.
- 与非压力组相比,80 mg/L As 压力组中甲基酶,超氧化物脱酶和谷氨的活性显著增加 (分别是1.10,1.09 和20.47 倍).
- 的物种化分析显示,V (As V) 是T. versicolor hyphae中占主导地位的物种,无论的压力如何,都表明As III的生物转化为As V.
结论:
- 特拉梅特 (Trametes versicolor) 通过增强抗氧化酶活动,特别是谷氨来减轻的毒性.
- 这种真菌通过将有毒的As III转化为不那么有害的As V和其他物种来排毒.
- T. versicolor表现出显著的耐受性和积累能力,将其定位为污染场所的可行生物积累器.
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