逆转移素R2维持了Drosophila的核糖体DNA的重复
Jonathan O Nelson1,2, Alyssa Slicko1,2, Yukiko M Yamashita1,2,3
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
概括
R2逆转移素通过修复DNA断裂,防止灭绝,维持Drosophila雄体中的核糖体DNA复制数. 这一必不可少的功能有利于宿主健康,并确保血统的生存.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 核糖体DNA (rDNA) 含有重复的基因拷贝,对细胞活力至关重要.
- 染色体内重组可以导致rDNA复制数的丧失,威胁到血统的维持.
- 抵消生殖系中rDNA损失的机制以前尚不清楚.
研究的目的:
- 调查R2逆转移素在保持Drosophila雄性生殖系中rDNA复制数的作用.
- 了解rDNA拷贝数是如何恢复的,以防止多代衰退和灭绝.
主要方法:
- 在Drosophila.中R2逆转移体的耗尽.
- 对rDNA复制数的维护和几代人的生育能力的分析.
- 在rDNA位置研究DNA断裂形成和修复机制.
主要成果:
- R2耗尽导致rDNA复制数维护有缺陷,导致生育率降低和灭绝.
- 由R2内核酶诱导的双链DNA断裂启动了rDNA复制数的恢复.
- 在rDNA复制处的DNA断裂的同质依赖性修复有助于rDNA恢复.
结论:
- R2逆转移子对于Drosophila雄性生殖系中恢复性rDNA拷贝数扩张至关重要.
- 活跃的逆转移子可以提供必要的宿主功能,有利于健康.
- 主体优势可能是可转移元素的选择性优势,解释了它们的流行.
更多相关视频
11:12Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
7.6K
10:26TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
Published on: September 10, 2015
17.9K
相关概念视频
LTR Retrotransposons
17.6K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.6K
Non-LTR Retrotransposons
11.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.6K
DNA-only Transposons
14.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.6K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
Overview of Transposition and Recombination
15.9K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.9K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
