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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

79
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.5K
Homologous Recombination02:31

Homologous Recombination

50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jul 28, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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克里斯普分析 (CRISPR-A):用于基因编辑的精确分析和模拟的平台.

Marta Sanvicente-García1, Albert García-Valiente1, Socayna Jouide2

  • 1Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.

PLoS computational biology
|May 30, 2023
PubMed
概括

克里斯普分析 (CRISPR-A) 是一种用于分析基因编辑结果的新工具. 它比现有方法提供更高的准确性和更广泛的功能,帮助各种基因编辑技术的实验设计和分析.

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相关实验视频

Last Updated: Jul 28, 2025

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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 目前的基因编辑特征化工具在量化编辑比例方面缺乏精度.
  • 对基因编辑结果的准确评估对于实验成功和临床应用至关重要.

研究的目的:

  • 开发一个全面和多功能基因组编辑网络应用程序和Nextflow管道,命名为CRISPR-Analytics (CRISPR-A).
  • 为了提高基因编辑分析的准确性和扩展功能.
  • 为了支持基因编辑实验设计和分析跨多种方法.

主要方法:

  • 在CRISPR-Analytics中包含基于模拟的噪声校正和spike-in校准的放大偏差减小.
  • 该工具具有先进的交互图形,用于数据可视化.
  • 一个模拟模块通过预测基因编辑结果来评估实验设计.

主要成果:

  • 与现有的基因编辑分析工具相比,CRISPR-A的准确性更高.
  • 该应用程序为敏感病例提供了强大的分析,包括临床样本和低编辑效率.
  • 克里斯普尔-A支持各种基因编辑技术,如基基编辑 (BE),原始编辑 (PE) 和同质导向修复 (HDR),而不需要特定的实验方法细节.

结论:

  • 克里斯普尔分析为基因编辑特征提供了一种多功能且准确的解决方案.
  • 该工具增强了实验设计和分析,提高了敏感应用的可靠性.
  • 克里斯普尔-A广泛适用于各种基因编辑策略,简化了分析工作流.