提高死体积:微样本的分析被稀释并用近红外追踪器进行校正
Dennis J Dietzen1,2, Connor J Blair1, Stephen M Roper1,2
1Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, United States.
The journal of applied laboratory medicine
|May 30, 2023
概括
将近红外染料稀释剂添加到小等离子体样本中,可以进行准确的分析,克服目前儿童实验室机器人系统的局限性. 这种方法可以实现微样本的自动处理.
科学领域:
- 临床化学 临床化学
- 实验室自动化 实验室自动化
- 生物医学工程 生物医学工程
背景情况:
- 机器人样本处理通常需要大量的液体体积,限制其在样本体积小的环境中使用,例如儿科实验室.
- 目前的解决方案包括重新设计硬件或手动处理亚毫升标本.
研究的目的:
- 评估使用含近红外染料 (IR820) 的稀释剂增加样本体积的可行性,以便在微样本中准确分析临床分析物.
- 评估稀释对各种常见实验室试验中的分析物回收和测定不精度的影响.
主要方法:
- 用含有IR820近红外染料的液体稀释等离子体样本.
- 用多种测试格式和波长分析稀释样本.
- 稀释样本中的分析物回收与干净样本进行了比较,并对IR820吸收率进行了校正.
主要成果:
- 在IR820吸收性校正后,平均分析回收率在93%至110%之间,与数学校正方法相似.
- 分析不精确度从整洁样本的2%变化到稀释到原始度的30%时的8%.
- 没有观察到染料的干扰,这表明稀释剂的广泛适用性和化学惰性.
结论:
- 用化学惰性,近红外标记物含有液体稀释微样品是一种可行的方法来增加样品体积.
- 这种方法有可能使小体积样本中的临床分析物的自动处理和测量成为可能.
- 该方法为处理有限样本体积的实验室提供了实用解决方案,例如儿科设置.
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