与线粒体相关的内分泌体网膜参与了线粒体功能障碍和由子中的铜引起的内分泌体网膜压力
Junjun Peng1, Xueyan Dai1, Huiqin Fan1
1Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Economic and Technological Development District, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Nanchang, 330045, Jiangxi, People's Republic of China.
概括
过度的铜通过破坏线粒体相关的ER膜 (MAM) 来损害细胞,导致线粒体功能障碍和北京的内分泌网膜压力.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 高水平的铜可以损害功能,但毒性的确切机制尚未完全理解.
- 线粒体功能障碍和内分泌网膜 (ER) 压力是重金属暴露的已知后果.
- 线粒体和ER通过线粒体关联的ER膜 (MAM) 相互连接,这表明它在细胞对毒素反应中的作用.
研究的目的:
- 研究线粒体相关ER膜 (MAM) 在铜诱导的毒性中的作用.
- 在过度接触铜的背景下,阐明线粒体和ER之间的交叉声.
- 了解铜诱导的损伤背后的分子机制.
主要方法:
- 144只北京被分为对照组和三个暴露于铜 (100,200,400毫克/公斤) 的组.
- 评估了MAM完整性,关键蛋白的同定位 (IP3R,VDAC1) 和与MAM相关的因素水平.
- 分析了线粒体功能标记物 (ATP,蛋白质表达) 和ER压力标记物 (蛋白质表达).
主要成果:
- 过量的铜破坏了MAM完整性,改变了MAM相关因子水平,表明MAM功能障碍.
- 铜暴露导致线粒体功能障碍,由降低的ATP水平和关键线粒体蛋白质的改变表达体现.
- 铜显著增加了ER压力标志物,包括Grp78,CRT和ATF4.
结论:
- 线粒体相关的ER膜 (MAM) 在铜诱导的线粒体功能障碍和ER压力中发挥着关键作用.
- 破坏MAM完整性是铜毒性的关键机制.
- 这些发现增强了对分子水平上铜诱导毒性的理解.
相关概念视频
Role of ER in the Secretory Pathway
5.5K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.5K
Smooth Endoplasmic Reticulum
5.9K
Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
5.9K
Export of Misfolded Proteins out of the ER
3.7K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.7K
Electron Transport Chain: Complex I and II
14.6K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.6K


