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急性寒冷压力通过CIRP-TLR4-IRE1信号通路诱导肠道损伤,在预启动肉中

Juanjuan Wu¹, Guiyao Li¹, Haoneng Guo¹

¹College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang, 330045, Jiangxi, China.

Molecular biology reports

|May 30, 2023

概括

肉的急性寒冷压力会通过激活冷诱导性RNA结合蛋白 (CIRP) -Toll-like受体4 (TLR4) -IRE1通路来损害肠道,从而导致内质网膜压力.

科学领域:

动物科学动物科学
生理学生理学生理学
分子生物学分子生物学

背景情况:

寒冷压力对肉来说是一个重大的环境挑战.
冷诱导型RNA结合蛋白 (CIRP) 通过托尔类受体4 (TLR4) 参与炎症反应.
对于CIRP在 brojler冷压中的确切作用仍在调查中.

研究的目的:

为了研究CIRP-TLR4-IRE1信号通路在遭受急性寒冷压力的肉小中的参与.
评估寒冷压力对肠道完整性和内质网膜压力标记物的影响.

主要方法:

360只肉被暴露在急性寒冷 (12±1°C) 中,持续时间为0,4,8或12小时.
测量包括腹部皮肤温度,基因表达 (CIRP,TLR4,IRE1通路,炎症因素,紧结蛋白) 和小肠形态.

主要成果:

寒冷暴露降低了腹部皮肤温度,并诱导了内细胞网膜应激 (ERS) 标志物 (IRE1,GRP78).
炎症因子 (IL-1β,IL-6,IL-10,TNF-α) 和紧结蛋白 (ZO-1,Occludin) 的基因表达被上调.
长时间暴露于寒冷会增加CIRP和TLR4的表达,同时降低小的高度和小/密室 (V/C) 比率.

结论:

急性寒冷压力会在肉小中引发细胞内膜网膜压力.
在冷应力过程中,CIRP-TLR4-IRE1信号通路受到上调.
寒冷压力导致小小肠的结构损伤.

关键词:

在CIRP中,CIRP是指CIRP.的肠道结构的肠道结构寒冷的压力是冷的压力.在此之前,ERS 基因表达基因表达基因表达

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