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Updated: Aug 3, 2026

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
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A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

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基于双螺旋微流体芯片和实时RPA方法的CTC中EGFR突变的快速检测

Wen-Man Li1, Xiao-Dong Ren1, Yu-Zhu Jiang2

  • 1Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, China.

Analytical and bioanalytical chemistry
|May 30, 2023
PubMed
概括

这项研究提出了一种快速,简单的方法来检测循环瘤细胞 (CTC) 中的癌症突变,使用微流体和实时RPA. 这一突破使得迅速,非侵入性癌症诊断和个性化治疗调整成为可能.

关键词:
循环的瘤细胞循环.检测EGFR突变的检测方法微流体芯片是一个微流体.非小细胞肺癌是非小细胞肺癌.复合酶聚合酶放大复合酶的放大.有针对性的药物治疗.

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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...