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相关概念视频

Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

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In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

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核化红细胞计数中的分析错误

Juliet E Brown1, Astrid Oscos-Snowball1, Natalie F Courtman1

  • 1U-Vet Werribee Animal Hospital and Faculty of Veterinary and Agricultural Science, University of Melbourne, Werribee, Victoria, Australia.

Veterinary clinical pathology
|May 30, 2023
PubMed
概括

狗和猫的自动核化红细胞 (NRBC) 计数与手动方法在低水平上显示出良好的一致性. 建议在较高的NRBC计数或特定样本条件下进行手动审查,以确保准确性.

科学领域:

  • 兽医血液学 兽医血液学
  • 临床病理学 临床病理学
  • 实验室自动化 实验室自动化

背景情况:

  • 在狗和猫外周血液中手动计数核红细胞 (NRBC) 是标准的,但劳动密集型.
  • 自动化分析仪提供了更高的效率,但NRBC和白细胞之间的准确区分仍然是一个挑战.
  • Sysmex XN-V 系列分析仪具有区分NRBC的潜力,但已经报告了分析错误.

研究的目的:

  • 在狗和猫血液样本中调查自动和手动NRBC计数之间的差异.
  • 识别和评估自动NRBC计数中分析错误的原因.

主要方法:

  • 具有高NRBC计数的血液样本的回顾性分析.
  • 将自动化NRBC计数与使用相关性和一致性分析的手动血液膜评估进行比较 (斯皮尔曼,帕辛-巴布洛克,布兰德-阿尔特曼).
  • 评估自动化方法的精度和手动计数的观察者间协议.

主要成果:

  • 自动和手动NRBC计数之间的良好一致性在狗和猫的NRBC数量≤1×109/L时被观察到.
  • 在≥1NRBC ×109/L的水平上,协议是不充分的,自动化方法显示了负比例差异.
  • 高血率,融合细胞碎片/白细胞云,以及早期红色素前体的存在,导致自动计数不准确. 在手动计数方面,观察者之间的差异很小 (平均CV为27.3%).
关键词:
在Sysmex中,我们使用的是Sysmex.对于WNR来说,这是一个很大的问题.这是一个错误的错误错误.这就是metarubricytosis.常态母细胞形成.标题:细胞分裂

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结论:

  • Sysmex XN-1000 V的WNR通道提供精确的NRBC计数,与手动计数的良好一致性在≤1NRBC ×109/L.
  • 手动血液膜检查对于具有≥1NRBC ×109/L,早期红色素前体或潜在的门错误的样本至关重要.
  • 特定的患者群体 (例如灰狗,脱水动物) 可能需要仔细解释自动NRBC结果.