相关实验视频
Updated: Jul 28, 2025

09:05
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
22.0K
CRISPR-GRANT:一个跨平台的图形分析工具,用于基于CRISPR的高吞吐量基因组编辑评估.
Huancheng Fu1,2, Ce Shan1, Fanchen Kang1
1Center for Growth Metabolism and Aging, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
BMC bioinformatics
|May 30, 2023
概括
CRISPR-GRANT是一个新的图形工具,为研究人员简化了CRISPR基因组编辑分析. 这种用户友好的软件允许从下一代测序数据中轻松进行indel分析,减少对生物信息学专业知识的需求.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 克里斯普尔/卡斯是一个强大的基因组编辑工具,具有显著的生物医学潜力.
- 在评估CRISPR/CasDNA编辑事件时,英德尔分析至关重要.
- 现有的独立分析工具往往需要生物信息学专业知识和命令行技能.
研究的目的:
- 开发一个用户友好的,独立的图形工具,用于CRISPR内部分析.
- 为湿实验室研究人员简化分析基因组编辑事件的过程.
- 为分析下一代测序数据提供可访问的解决方案.
主要方法:
- 开发CRISPR-GRANT,一个图形用户界面 (GUI) 工具.
- 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性 (Linux,Windows,macOS). 跨平台的兼容性
- 单个/聚合的安普利康和全基因组测序数据的一步分析.
主要成果:
- CRISPR-GRANT提供了一个简单的点击运行界面,非常适合新手用户.
- 该工具不需要对测序数据进行预处理.
- 与现有工具相比,CRISPR-GRANT的运行时间更短.
结论:
- 克里斯普尔-格兰特显著降低了湿实验室研究人员在进行内部分析方面的障碍.
- 它是CRISPR工具包中一个有价值的补充,用于分析大型下一代测序数据集.
- 该软件可以免费下载和使用.
相关概念视频
CRISPR/Cas9 Genome Editing
79
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
79
CRISPR and crRNAs
17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
CRISPR
52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.5K
Homologous Recombination
50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.7K

