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大麻种子:一种潜在的与子交叉反应的过敏原来源
Princy Beriziky1, Mehdi Cherkaoui2, Laura Linxe3
1INRAE, UR BIA, F-44316, Nantes, France; CHU d'Angers, unité d'allergologie, Angers, France.
Food research international (Ottawa, Ont.)
|May 31, 2023
概括
大麻种子过敏症正在出现. 研究人员将vicilins和edestins确定为潜在的麻过敏原,这表明在一些人中与子过敏有交叉反应.
科学领域:
- 食品科学 食品科学 食品科学
- 过敏学 过敏学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 大麻sativa (大麻) 的暴露正在增加,导致对包括大麻种子在内的各种植物部位的鉴定过敏.
- 大麻种子中的主要过敏原尚未完全确定,尽管已知存在种子储存蛋白质.
研究的目的:
- 为了研究大麻种子储存蛋白质的过敏性.
- 探索大麻种子蛋白与其他种子/坚果之间的潜在交叉反应.
主要方法:
- 从大麻种子中使用缓冲剂增加盐度 (H2O,T2,T3) 的顺序提取蛋白质.
- 用患者血清 (对大麻或其他种子/坚果敏感) 进行免疫血栓和ELISA.
- 蛋白质组学分析以确定特定的蛋白质.
主要成果:
- 被确定为潜在的麻种子过敏原的维西林和埃德斯.
- 在子敏感度和对大麻种子提取物的反应性之间发现了相关性 (T3).
- 抑制ELISA测试显示,麻种子提取物对子提取物结合IgE的抑制率为25-30%,表明交叉反应性.
结论:
- 维西林和埃德斯被确定为潜在的麻种子过敏原.
- 凸显了大麻种子和子蛋白之间可能的交叉反应.
- 需要进一步调查以确定对子过敏个体的临床相关性.
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