在水牛精液扩展剂中的埃普西隆聚氨酸:在减少抗生素耐药性的发展方面迈出了一步
Usha Yadav1,2, Ravi Dutt2, Krishna Bansal3
1Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, India.
Reproduction in domestic animals = Zuchthygiene
|May 31, 2023
概括
西隆聚氨酸 (Ɛ-PL) 在水牛精液扩展剂中有效取代抗生素,保持精子质量,并在冷保存期间提供抗菌和抗氧化功效.
科学领域:
- 兽医繁殖 兽医繁殖 兽医繁殖
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 在精液扩展剂中使用抗生素有助于抗菌素耐药性.
- 在动物人工授精中,需要安全有效的抗生素替代品.
研究的目的:
- 为了评估epsilon-polylysine (Ɛ-PL) 作为牛精液扩展器中抗生素的替代品.
- 评估 Ɛ-PL 对水牛精子的抗菌和冷保护作用.
主要方法:
- 牛精液射精被冷保存,使用带有抗生素的扩展剂或不同度的 Ɛ-PL.
- 进行了抗微生物敏感性测试和殖民地形成单位 (CFU) /mL计数.
- 分析了精子运动性,动力学参数,体完整性,线粒体膜潜力和超氧化离子产生.
主要成果:
- Ɛ-PL证明了对水牛精液微生物的抗微生物疗效,与菌素相当.
- 冷保存的水牛精子质量,包括运动性和动力学,没有受到 Ɛ-PL. 的负面影响.
- Ɛ-PL补充剂增强了精子和线粒体膜的潜力,并减少了超氧化离子的产生.
结论:
- -聚氨酸 (Ɛ-PL) 是水牛精液扩展器中抗生素的可行替代品.
- 对于 Ɛ-PL,0.64 g/L 的最佳度为精子冷保存提供了抗菌和抗氧化功效.
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