相关实验视频

Updated: Jul 28, 2025

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

显著增加了内镜的细菌污染,夜间和周末的储存时间显著增加

Simone Vieira Toledo Guadagnin¹, Dayane de Melo Costa^2,3, Mariusa Gomes Borges Primo¹

¹Clinics Hospital, Federal University of Goias, Goiânia, Goias, Brazil.

Journal of gastroenterology and hepatology

|May 31, 2023

概括

在没有强制空气干燥 (FAD) 柜存储的柔性胃镜中,细菌污染增加. 在非FAD柜中储存时间较长导致污染较高,这表明生物膜的繁殖.

科学领域:

感染控制感染控制
微生物学微生物学
医疗设备再加工医疗设备再加工

背景情况:

强制空气干燥 (FAD) 柜被推用于重新加工的内镜.
在许多医疗保健机构中,财务限制限制了FAD柜的使用.
替代储存方法需要评估细菌污染风险.

研究的目的:

在储存后在柔性胃镜 (FG) 中评估细菌污染.
为了评估存储在一个柜子里,有过的空气和紫外线,但没有FAD.
为了确定储存时间对污染水平的影响.

主要方法:

在再加工后0,12小时和60小时采样了8个柔性胃镜.
道样本采集采用冲刷冲刷冲洗方法.
使用自动化方法进行了细菌鉴定和抗生素耐药性测试.

主要成果:

与零时相比,12小时和60小时的细菌污染明显高于零时.
60小时后,格拉姆阴性杆构成了大多数细菌 (52.3%).
在60小时内隔离的65%细菌表现出抗生素耐药性.

结论:

在非FAD柜中存储的重新加工的柔性胃镜显示细菌污染增加.
长时间储存 (60小时) 会加剧污染,可能是由于生物膜生长.
需要基于现有柜技术的基于证据的储存时间指导方针.

关键词:

消毒消毒消毒消毒胃肠道内镜,胃肠道内镜设备的污染,生物膜.

更多相关视频

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

相关概念视频

Key Techniques in Microbiology

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...