微封装Bacillus subtilis和麦β-葡萄糖,并将其作为鱼料中的共生菌应用
Angélica Priscila do Carmo Alves1, Amanda do Carmo Alves2, Rodney Alexandre Ferreira Rodrigues3
1Departamento de Zootecnia, Escola Superior de Agricultura Luiz de Queiroz [ESALQ], Universidade de São Paulo [USP], Piracicaba, São Paulo, Brazil.
Journal of microencapsulation
|May 31, 2023
概括
使用喷雾干燥将Bacillus subtilis与麦β-葡萄糖微封装显著提高了其在储存和模拟胃液暴露期间的存活率. 这种方法为益生菌应用提供了潜力,特别是在鱼料中.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 像Bacillus subtilis这样的益生菌需要对恶劣的储存和胃肠道条件进行保护,以便有效的输送.
- 麦β-葡萄糖是一种已知的生物聚合物,具有潜在的保护性质.
研究的目的:
- 评估使用麦β-葡萄糖和喷雾干燥来保护细菌细菌的微封装疗效.
- 在储存和模拟胃条件下评估微封装B. subtilis的存活率.
主要方法:
- 用麦β-葡萄糖喷雾干燥Bacillus subtilis.使用麦β-葡萄糖.
- 微囊的特征 (形态,大小,封装效率,湿度,水活性,湿度,泽塔潜力).
- 免费和微封装细胞暴露于模拟的胃液 (SGF) 和在不同温度下存储90天.
主要成果:
- 微囊的颗粒大小为1.5 ± 0.34μm,封装效率为77.9 ± 3.06%.
- 微封装的B. subtilis在SGF暴露后与自由细胞 (7.6 ± 0.06 log CFU mL−1) 相比,显示了更高的生存率 (8.4 ± 0.07 log CFU mL−1).
- 微封装细胞在储存90天后保持活力超过6个日志单位,与自由细胞不同.
结论:
- 用麦β-葡萄糖喷雾干燥是一种有效的方法来保护Bacillus subtilis的生存能力.
- 微封装策略在储存和模拟胃条件下改善益生菌的存活率.
- 这种技术对B. subtilis在鱼配方中的应用有希望.
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