在植物中通过MoonTag可编程转录激活器有效激活基因
J Armando Casas-Mollano1,2, Matthew H Zinselmeier1,2,3, Adam Sychla1,2
1Department of Biochemistry, Molecular Biology, and Biophysics and Biotechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA.
Nucleic acids research
|May 31, 2023
概括
研究人员开发了MoonTag,这是一个新的CRISPR/Cas转录激活剂. 月球标签有效地诱导植物的基因表达,并显示稳定的表达,没有负面影响,为植物生物技术提供了一个有前途的工具.
科学领域:
- 分子生物学分子生物学
- 植物生物技术 植物生物技术
- 基因规则 基因规则
背景情况:
- 基于CRISPR/Cas的转录激活剂能够在各种生物体中实现向基因表达.
- 第二代SunTag系统招募多个激活域,但在某些物种中面临表达挑战.
- 转录激活剂的稳定表达对于转基因植物有效的基因调节至关重要.
研究的目的:
- 开发一种基于CRISPR/Cas的新型转录激活剂MoonTag,在转基因植物中改善稳定表达.
- 评估MoonTag在诱导各种植物物种高水平转录中的有效性.
- 为了评估MoonTag在不同温度的性能,以潜在的现场应用.
主要方法:
- 设计和制造MoonTag系统,一个修改的CRISPR/Cas激活器.
- 用 MoonTag 组件稳定转化各种植物物种 (单和双).
- 使用MoonTag.对报告者和内源基因表达水平的量化.
- 在不同的温度条件下评估MoonTag成分表达和植物表型.
主要成果:
- MoonTag成功地在所有测试的植物物种中诱导了高水平的转录,包括Setaria,Arabidopsis和西红.
- 在转基因植物中,MoonTag成分在高水平上稳定表达,没有可观察到的有害影响.
- 通过MoonTag激活基因在各种温度范围内是功能性的,表明了强度.
- 月球标签证明了Setaria中报告基因和内源基因的有效激活.
结论:
- 月球标签代表了基于CRISPR/Cas的植物转录激活的重大进步,克服了以前系统的局限性.
- 该系统表现出强大的性能,高效率和稳定的表达,使其适用于植物生物技术中的各种应用.
- MoonTag在不同温度的功能表明,它有可能在农业和作物改进中成功地进行现场应用.
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