硫烯酸CLEL6抑制了Arabidopsis thaliana中的反素生物合成
Eric Bühler1, Elisa Fahrbach1, Andreas Schaller1
1Department of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim, Stuttgart 70593, Germany.
Plant physiology
|May 31, 2023
概括
该CLEL6作为一个负调节者,对阿拉比多普西斯幼苗中安托素生物合成. 它的功能需要加工和硫化,它通过RGI受体发出信号,以控制压力期间的色素积累.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物信号通道的信号通道.
- 类激素研究研究 类激素研究
背景情况:
- 翻译后修改的调节了植物的应激反应.
- 安西安素是植物颜料,参与了应激耐受性.
研究的目的:
- 确定Arabidopsis中安托氨酸生物合成的调节者.
- 阐明CLEL6在植物应激反应中的作用.
主要方法:
- 阿拉比多普西斯突变的遗传分析 (sbt6.1,tpst,RGI五重突变).
- 对的加工和修改进行生物化学分析 (蛋白质分解裂变,氨酸硫化).
- 基因表达分析的安托西亚宁生物合成途径基因.
- 在轻度应力下,对安托西亚宁积累的表型分析.
主要成果:
- 克莱尔6可以负面调节以化和光应激的阿拉比多普西斯幼苗中的安托素生物合成.
- 通过SBT6.1进行CLEL6加工和通过TPST进行氨酸硫化对其功能至关重要.
- 在SBT6.1或TPST中功能丧失的突变体在轻度应激下表现出增加的安托西亚宁积累.
- 通过RGI家族的氨酸丰富的重复受体类激酶来调节CLEL6信号传递.
结论:
- 克莱尔6作为一种小硫酸激素起作用,抑制了氨酸生物合成.
- 在调节植物对光应激反应方面,CLEL6-RGI信号通路起着至关重要的作用.
- 在压力条件下,CLEL6和相关的硫作为负调节剂,限制在压力条件下安东的积累.
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