室用于增强微流体液滴的化和成像
Nicolas Lobato-Dauzier1,2, Robin Deteix1,2, Guillaume Gines3
1LIMMS, CNRS-Institute of Industrial Science, IRL 2820, University of Tokyo, Tokyo 153-8505, Japan. genot@iis.u-tokyo.ac.jp.
Lab on a chip
|May 31, 2023
概括
(Si) 室为滴滴微流体提供了卓越的性能,克服了聚二甲基素 (PDMS) 的局限性. 这些Si室可实现高通量成像和精确的温度控制,用于先进的生物和物理科学应用.
科学领域:
- 微流体学 微流体学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 滴滴微流体对于数字测试,单细胞测序和生命科学中的定向进化至关重要.
- 当前的微流体设备经常使用聚甲基素 (PDMS),它具有低于最佳的光学,热和机械性能,用于滴滴成像和化.
- PDMS的局限性阻碍了高通量滴滴分析和精确的环境控制.
研究的目的:
- 研究 (Si) 作为液滴微流体室的替代材料.
- 在滴滴处理,成像和热控制方面评估Si室的性能.
- 为了证明Si室在复杂,高通量滴滴基试验中的实用性.
主要方法:
- (Si) 滴滴室的制造,其设计精简,以实现可重复性.
- 用于滴滴包装,稳定性,光学性能和热控制的Si腔室的表征.
- 在多重数字测试中应用Si腔室,用于成像大约100万滴.
- 在一个苛刻的测试中利用Si腔室来绘制Michaelis-Menten常数的热依赖,使用大约15万滴.
主要成果:
- Si腔室可以实现晶体,不移动的单层滴滴包装,防止蒸发和松.
- 室显著增强光子的收集,并提供精确的温度控制滴.
- 高通量成像达到100万滴,其采集率与领先的线式成像方法相美.
- 成功演示了一种要求很高的测试测绘酶动力学在一个大的滴滴阵列.
结论:
- (Si) 是滴滴微流体室的理想材料,与PDMS相比,它具有显著的优势.
- Si腔室可提供强大的,高性能滴滴操纵,成像和精确的热管理.
- 室的精简设计和优越性质使它们成为推进滴滴微流体学的宝贵补充材料.
相关概念视频
01:18Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...