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基于CRISPR-Cas9的SlHyPRP1蛋白精确工程,以实现番茄多重压力耐受性

Mil Thi Tran^1,2,3, Geon Hui Son¹, Young Jong Song¹

¹Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea.

Frontiers in plant science

|May 31, 2023

概括

番茄中的CRISPR-Cas9基因编辑精确地删除了SlHyPRP1基因的域,创造了对盐度,热量和干旱压力的耐受性增强的植物,并提高了对细菌病原体的抵抗力.

科学领域:

植物科学植物科学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

CRISPR-Cas9基因组编辑是植物育种的强大工具.
番茄基因SlHyPRP1,编码一种富含氨酸的混合蛋白1,对盐的压力反应产生负面调节.
之前的研究精确地使用CRISPR-Cas9.9消除了SlHyPRP1的功能域 (富含proline的域和八个cysteine动机).

研究的目的:

为了表征CRISPR-Cas9编辑的番茄线,以应对多重压力耐受性.
评估SlHyPRP1域编辑基因在番茄育种中的潜力,以提高弹性.

主要方法:

为了精确地编辑SlHyPRP1.1,使用CRISPR-Cas9多重复合方法.
在非生物 (热量,透,干旱) 和生物 (细菌,真菌) 压力下编辑的西红线的表型特征.
在编辑型和野生型植物中对细菌病原体生长的定量评估.

主要成果:

SlHyPRP1域移除变体 (8CM,PRD) 赋予了中度的热应激耐受性.
移除PRD和8CM的变种在透应激 (曼尼托尔) 下显示出改善的发芽.
PR1v1系列在断水 (干旱压力) 后表现出增强的生存率.
编辑后的线条显示,对Pseudomonas syringae pv.的敏感性显著降低. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto) DC3000. 番茄 (Pto)
编辑的等位基因增加了对Fusarium oxysporum f. sp.的敏感性. 这就是Lycopersici的意思.

结论:

精确的基于CRISPR-Cas9的SlHyPRP1域编辑产生了多重耐压等位基因.
这些编辑的等位基因为开发弹性番茄品种提供了宝贵的遗传资源.
虽然对某些压力有好处,但在抗病能力方面的潜在权衡需要考虑.

关键词:

克里斯普尔-Cas9是什么意思这就是HyPRP1的原因.无生物压力是无生物压力.生物压力是生物压力.热应力耐受性耐热应力耐受性多重压力耐受性多重压力耐受性

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