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使用纳米孔MinION和Illumina MiSeq测序对新生儿肠道微生物组进行分析

Teahyen Cha¹, Hoo Hugo Kim², Jihyun Keum³

¹Department of Pediatrics, Hanyang University College of Medicine, Seoul, Republic of Korea.

Frontiers in microbiology

|May 31, 2023

概括

牛津纳米孔技术 (ONT) 测序准确地描述了婴儿肠道微生物组,揭示了早产婴儿和早产婴儿之间的明显差异. 在新生儿中,ONT显示了临床病原体检测的潜力.

科学领域:

微生物学和基因组学微生物学和基因组学
新生儿研究新生儿研究
生物信息学是一种生物信息学.

背景情况:

婴儿肠道微生物组的发展对健康至关重要.
产前和产后的婴儿表现出不同的微生物殖民模式.
准确的微生物组分析对于了解婴儿健康和疾病至关重要.

研究的目的:

为了比较第三代牛津纳米孔技术 (ONT) 测序与已建立的Illumina测序用于婴儿肠道微生物组分析.
确定肠道微生物组组成的差异以及早产婴儿和早产婴儿之间的多样性.
评估ONT测序在新生儿临床环境中用于病原体检测的潜力.

主要方法:

从51名早产婴儿和早产婴儿的便样本在生命的第7天和第28天收集.
使用ONT (长读) 和Illumina (短读) 平台进行了16S rRNA基因测序.
生物信息分析被用来比较分类学概况,α-多样性和β-多样性.

主要成果:

无论是ONT还是Illumina测序,在属级别上都产生了可比的分类学概况.
在28天的早产婴儿和早产婴儿之间观察到肠道微生物组合的显著差异.
与早产婴儿相比,预产婴儿的有益细菌 (例如Bifidobacterium,Lactobacillus) 和α多样性增加,这些婴儿的病原性细菌 (例如Klebsiella) 患病率更高.

结论:

ONT测序是一种可靠的方法,用于分析婴儿肠道微生物组,与Illumina测序高度相关.
产前和产后的婴儿表现出不同的肠道微生物组轨迹,这对新生儿健康有影响.
在新生儿临床环境中,ONT测序显示出快速和准确的病原体检测潜力.

关键词:

在Illumina MiSeq®上使用.纳米孔小型化我们的肠道微生物组.新生儿们的新生儿过早出生的婴儿早产.

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