对营养密度的适应性反应:通过脏的转录组分析揭示了免疫功能变化
Yan Zhou1, Dingguo Cao1, Jie Liu1
1Poultry Breeding Engineering Technology Center of Shandong, Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
Frontiers in immunology
|May 31, 2023
概括
在中减少食代谢能量 (ME) 和原蛋白 (CP) 显著降低了IgM水平和调高了免疫基因,可能导致炎症. 这项研究探讨了脏对营养密度变化的免疫反应的分子机制.
科学领域:
- 禽类的营养 养
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 料成本占家禽生产成本的65-70%.
- 饮食调整,包括减少原蛋白 (CP) 和代谢能 (ME),旨在优化的营养和生产.
- 关于饮食限制如何影响的免疫细胞功能机制的研究有限.
研究的目的:
- 研究可代谢能量 (ME) 和原蛋白 (CP) 限制对血清免疫球蛋白和脏免疫基因表达的影响.
- 分析脏免疫反应背后的分子机制,以对不同的食营养密度进行分析.
主要方法:
- 216只YS-909肉接受了9种饮食治疗 (低,标准和高ME或CP水平的3x3因数排列).
- 血清免疫球蛋白使用酶相关免疫吸收定量 (ELISA) 进行测量.
- 脏免疫基因表达通过使用RNA测序的转录基因分析来分析.
主要成果:
- 与其他组相比,在养低ME饮食的肉中观察到显著较低的IgM水平 (p <0.05).
- 与免疫相关的基因 (例如,MX1,USP18,TLR4,IFNG,IL18) 在饮食营养密度下降时显著上调.
- 这些基因的上调表明,由营养限制引起的潜在炎症状态.
结论:
- 饮食ME和CP限制影响肉的幽默免疫力 (IgM水平).
- 降低营养密度会触发脏中特定免疫相关基因的上调.
- 这项研究提供了关于脏免疫反应对禽类饮食营养密度变化的分子机制的见解.
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