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circSKA3通过miR-1238和甲基化促进结直肠癌转移

Yonghuan Mao^1,2, Ji Miao¹, Ling Xi³

¹Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, 210008, China.

Molecular and cellular biochemistry

|May 31, 2023

概括

像cirSKA3这样的循环RNA (circRNAs) 在结直肠癌 (CRC) 中被上调. 通过调节miR-1238 / YTHDF2 / STAT5A通路,CircSKA3促进CRC细胞的生长和入侵,提供潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

结肠直肠癌 (CRC) 是全球癌症死亡的主要原因.
循环RNAs (circRNAs) 是一种非编码RNA的类别,涉及到各种癌症,但它们在CRC中的特定作用在很大程度上是未知的.

研究的目的:

为了研究circRNAs在结直肠癌发展中的作用.
确定参与CRC进展的特定circRNA并阐明它们的潜在机制.

主要方法:

对GEO数据库进行生物信息分析,以识别差异表达的circRNAs.
定量PCR用于验证circRNA表达水平.
细胞增殖,迁移和入侵试验 (CCK-8,划伤,Transwell).
双露西法酶记者测定和RNA免疫沉降 (RIP) 测定以确定分子相互作用.

主要成果:

在结直肠癌组织中,CircSKA3被显著上调,促进了CRC细胞的增殖,迁移和入侵.
CircSKA3作为miR-1238的海绵,导致YTHDF2表达的增加.
过度表达YTHDF2逆转了miR-1238的抑制作用,并且发现YTHDF2降低了STAT5A甲基化.

结论:

循环SKA3在CRC上调节,并通过circSKA3/miR-1238/YTHDF2/STAT5A轴驱动瘤的进展.
这一途径代表了结直肠癌治疗的新治疗标.

关键词:

在CRC中,CRC就是CRC.甲基化甲基化甲基化这就是CircSKA3的意义.在 miR-12388 里面,你会看到 miR-1238 的位置.

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