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传统的HE染色与两种特殊的染色方法相结合,以识别高度分解的形尸体的死亡前刺伤
Zeyu Chen1, Xiaojun Yu2, Yinglin Zhu1
1Department of Forensic Pathology, National Key Disciplines, Collaborative and Creative center, Shantou University Medical College, Shantou, Guangdong Province, P.R. China.
Forensic science, medicine, and pathology
|May 31, 2023
概括
在腐烂的尸体上识别死前刺伤是具有挑战性的. 结合血氧林-素 (HE) 染色与庞索-维多利亚蓝色B (P-VB) 和马森染色,可以改善伤害和出血的可视化.
科学领域:
- 法医病理学 法医病理学
- 组织病理学 组织病理学
- 法医科学 法医科学 法医科学
背景情况:
- 在腐烂的遗骸上识别死前的伤害对于法医调查至关重要.
- 传统的组织学方法可能不足以可视化晚期分解中创伤的微妙迹象.
研究的目的:
- 评估组合组织学染色技术的有效性,以识别高度腐烂的,受损的尸体中的死前刺伤.
- 要区分死后的变化和死前的伤害相关出血.
主要方法:
- 从一个腐烂的尸体中取出部组织样本的组织学分析.
- 应用传统的血素-素 (HE) 染色方法.
- 应用Ponceau-Victoria蓝B (P-VB) 染色的方法.
- 马森三色彩染色的应用.
主要成果:
- 血氧氨酸 (HE) 染色只提供了基本的形态轮,出血和结缔组织都呈粉红色.
- 庞索-维多利亚蓝色B (P-VB) 染色明显区分出血 (黄色-绿色) 和结缔组织 (色-红色).
- 马森染色有效地区分出血 (紫红色) 和结缔组织 (深蓝色).
结论:
- HE,P-VB和马森染色的组合显著提高了在已分解的遗体中识别死前刺伤和相关出血的可能性.
- 这些增强的组织学技术为涉及严重腐烂和肢解尸体的法医病例工作提供了更可靠的方法.
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