在Sphingomonas lycopersici sp.中发现的 nov.,从番茄根球土壤中分离出来
Ruixiang Gao1,2, Honghong Dong1, Yang Liu1
1Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
概括
从番茄土壤中分离出了两种新型细菌菌株,即Sphingomonas lycopersici MMSM20和MMSM24. 这些促进植物生长的细菌产生的英多尔-3-酸和 siderophore,提供了潜在的农业应用.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 细菌学 细菌学是一门学科.
背景情况:
- 树球细菌在植物生长和健康中起着至关重要的作用.
- 类 * Sphingomonas * 包括具有不同代谢能力和生态作用的物种.
- 对新型植物相关细菌的隔离和表征对于理解微生物群落及其功能至关重要.
研究的目的:
- 从番茄根球中分离和描述具有促进植物生长潜力的新型细菌菌株.
- 通过多相性表征,包括基因组和遗传学分析,确定分离的菌株的分类地位.
- 根据不同的基因型和表型属性,在*Sphingomonas*属中提出一种新物种.
主要方法:
- 从番茄根球土壤中分离的细菌.土壤.
- 基因基因分析基于16S rRNA基因序列和92个核心基因.
- 基因组分析包括平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
- 类型表征,包括脂肪酸分析,子分析和极性脂质分析.
主要成果:
- 两种菌株,MMSM20T和MMSM24,被确定为有氧的,格拉姆染色阴性杆,能够产生印-3-酸和 siderophore.
- 遗传学分析将这些菌株置于 * Sphingomonas * 属内,与 * Sphingomonas jeddahensis * * Sphingomonas mucosissima * * 和 * Sphingomonas dokdonensis * * 密切相关.
- ANI和dDDH值证实MMSM20T和MMSM24菌株与已知物种相同但不同,支持它们被归类为新物种.
- 基因组DNA的G+C含量,主要脂肪酸,占主导地位的和极性脂质配置文件被确定为新型菌株.
结论:
- 隔离的菌株MMSM20T和MMSM24代表了Sphingomonas*属的一个新物种.
- 这种新物种的拟议名称是 *Sphingomonas lycopersici* sp. 新的,MMSM20T作为类型菌株.
- 这一发现有助于了解鱼菌的多样性及其在植物相关微生物群落中的潜在作用.
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