相互作用的细菌素对MreB-less多细胞Rhodomicrobium vannielii的细胞形状产生影响
Pia Richter1,2, Brigitte Melzer1,3, Frank D Müller1
1Department of Microbiology, University of Bayreuth, Bayreuth, Germany.
PLoS genetics
|May 31, 2023
概括
细菌素在MreB-less细菌的复杂细胞形状中发挥着至关重要的作用,如Rhodomicrobium vannielii. 这项研究揭示了它们在细胞生长中的重要功能,并提出了一种新型的杂交细菌素,将R. vannielii确立为 prokaryotic 发育的新模型.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 细菌细胞骨架的细胞骨架
背景情况:
- 大多数细菌都使用MreB细胞骨来形成棒状的细胞形状.
- 非棒形状通常需要辅助的细胞骨元素,如细菌素.
- 在MreB-less,尖端生长的细菌中,细菌素的功能仍然在很大程度上是未知的.
研究的目的:
- 为了研究细菌素在Rhodomicrobium vannielii的形态发生中的作用,一个MreB-less细菌.
- 建立对R. vannielii的遗传访问以研究细胞发育.
- 了解如何形成复杂的细胞形状没有MreB.
主要方法:
- 针对R. vannielii的向性突变发生.
- 光显微镜观察蛋白质定位和细胞形态.
- 蛋白质相互作用研究.
- 类甘油的合并分析.
主要成果:
- R. vannielii的细菌素局部存在于叶生长区.
- 一种必不可少的巴克托菲林是正确的形形成所需的.
- 鉴定出一种能够共聚合的新型杂交细菌素 (bactofilin).
- R. vannielii已经成功地进行了基因改造.
结论:
- 细菌素对于MreB-less细菌的复杂形态生成至关重要.
- R. vannielii 作为研究 prokaryotic 细胞发育和生长控制的有价值的新模型.
- 这些发现揭示了在没有MreB的情况下用于细菌细胞形状的替代机制.
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