/多醇基物质调节抗生素耐药基因的等离子体转移:氧化应激和能量支持之间的平衡
Congcong Liu1, Xiangyu Zhu1, Luhua You2
1Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China.
Water research
|May 31, 2023
概括
通过/多醇基物质 (PFAS) 影响抗生素耐药性基因 (ARG) 转移. 低度的PFAS促进了等离子体结合,而高度则通过影响细胞能量和L-氨酸水平来抑制它.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 抗生素耐药性基因 (ARG) 通过等离子体结合传播,这是一个公共卫生问题.
- 透/多醇基物质 (PFAS) 是具有已知的毒性作用的新兴污染物.
- PFAS对ARG传播的影响在很大程度上是未知的.
研究的目的:
- 研究包括PFOA,PFDoA和GenX在内的PFAS对等离子体结合的作用.
- 阐明PFAS介导的ARG转移调节的基本机制.
主要方法:
- 对大肠杆菌暴露于不同度的PFOA,PFDoA和GenX.
- 质粒结合频率的量化.
- 测量活性氧物种 (ROS),细胞膜透性,ATP含量和细胞内L-氨酸水平.
- 相关基因表达的转录组分析.
主要成果:
- 低度 (0.01,0.1 mg/L) 的PFOA,PFDoA和GenX促进了等离子体RP4的结合转移.
- 高度 (1.10 mg/L) 抑制了结合,与减少ATP和L-氨酸耗尽有关.
- 在低度和高度下,PFOA暴露增加了ROS的产生和膜透性.
- 在高PFOA度组中,氨酸补充恢复了结合.
结论:
- PFAS对等离子体结合具有双重作用,在低剂量时促进,在高剂量时抑制.
- 机制包括ROS的过度生产,变化的膜透性,能量耗尽和L-氨酸缺乏.
- 调查结果强调了PFAS在ARG传播中的作用,并为环境风险管理策略提供了信息.
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