普鲁士蓝色纳米颗粒:一种FDA批准的物质,在生理pH值下可能迅速降解
Lavinia Doveri1, Giacomo Dacarro1, Yuri Antonio Diaz Fernandez1
1Dipartimento di Chimica, Università degli Studi di Pavia, viale Taramelli, 12, 27100 Pavia, Italy.
Colloids and surfaces. B, Biointerfaces
|May 31, 2023
概括
普鲁士蓝色纳米颗粒 (PBnp) 在pH值7及以上不稳定,迅速降解. 然而,聚合物涂层或血清蛋白冠状体可以在生理条件下稳定PBnp.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 普鲁士蓝 (PB) 是FDA批准的口服药物,在酸性环境中稳定.
- 建议在生理条件 (pH 7.4) 下使用PB纳米粒子 (PBnp).
- 关于在生理pH值下PBnp的稳定性存在相互矛盾的报道.
研究的目的:
- 确定普鲁士蓝纳米粒子 (PBnp) 在生理pH的稳定性.
- 在生理条件下研究PBnp的稳定策略.
- 评估PBnp对细胞活性的影响.
主要方法:
- 用光谱分析来监测PBnp降解.
- 聚合物涂层 (PVP) 的评价,用于PBnp稳定.
- 在血清中评估蛋白质冠状形成.
- 使用EA.hy926,NCI-H1299和A549细胞系进行细胞活力测定.
主要成果:
- PBnp在pH≥7时本质上不稳定,其700nm吸收带的损失表明这一点.
- 聚烯 (PVP) 涂层在pH 7.4下保护PBnp超过24小时.
- 血清在PBnp上迅速形成蛋白质冠状,防止降解.
- 在各种条件下,PBnp治疗没有影响测试细胞系的活力.
结论:
- 普鲁士蓝色纳米粒子在中性和性pH下由于Fe3+与氧化离子的复合而降解.
- 聚合物涂层和蛋白质冠状形成是稳定PBnp在生理环境中的有效策略.
- PBnp具有良好的生物相容性,没有观察到对细胞活性的影响.
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