在亚致死的抗生素暴露下,细胞内和细胞外抗生素耐药性基因的不同剂量反应模式
Lele Liu1, Xinyi Zou1, Yifan Gao1
1College of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211816, China.
Ecotoxicology and environmental safety
|May 31, 2023
概括
抗生素耐药性基因 (ARG) 在水和生物膜中传播的方式不同. 细胞内ARG随着抗生素度的增加而增加,而细胞外ARG则减少,影响环境风险评估.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生态毒理学 生态毒理学
背景情况:
- 抗生素是抗生素耐药性基因 (ARG) 传播的主要驱动因素.
- 有限的研究存在于ARG剂量-反应关系在亚致死的抗生素度.
- 了解这些关系对于对抗生素的环境风险评估至关重要.
研究的目的:
- 研究细胞内 (iARG) 和细胞外 (eARG) 抗生素耐药性基因的剂量反应关系.
- 在不同抗生素度和频率下,检查ARG在水和生物膜中的传播.
- 评估抗生素混合物和剂量策略对ARG传播的联合影响.
主要方法:
- 构建微观世界来模拟环境条件.
- 微生物群落暴露在各种抗生素度 (1-100μg/L) 和频率的范围内.
- 在水和生物膜矩阵中量化细胞内和细胞外的ARG.
- 对剂量反应,协同效应/对抗效应以及频率依赖传播的分析.
主要成果:
- 细胞外ARG在水中更为丰富,而细胞内ARG在生物膜中占主导地位.
- 由于增强的选择,细胞内ARG与抗生素度增加.
- 细胞外ARG随着抗生素度更高而减少,因为分泌被抑制.
- 联合抗生素暴露显示出对iARG的协同作用和对eARG的对抗作用.
- 一次剂量有利于iARG,而分量剂量有利于eARG.
结论:
- 抗生素度和剂量策略显著影响细胞内和细胞外ARGs的传播动态.
- 对iARG和eARG的差异化剂量反应关系凸显了ARG传播的复杂性.
- 研究结果为评估抗生素耐药性带来的环境风险提供了关键的见解.
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