一个病原体效应器在共生真菌中多样化
Albin Teulet1, Clément Quan1, Edouard Evangelisti1
1Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, UK.
The New phytologist
|May 31, 2023
概括
鉴定出真菌病原体和共生状菌根菌 (AM) 菌因子之间的结构相似性. 这些发现表明,双域 (FOLD) 蛋白质在各种真菌的植物殖民中具有保留作用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病原体相互作用
- 共生真菌是一种共生性真菌.
背景情况:
- 病原性真菌分泌效应蛋白来抑制宿主免疫力.
- 在共生真菌中也发现了效应体,比如树状菌根 (AM) 菌,它们与植物形成营养共生.
- 基于序列的病原体和AM真菌之间的效应因子的比较是具有挑战性的,因为序列的多样性很高.
研究的目的:
- 为了确定共生菌和病原菌之间共享的序列分离但结构相似的效应蛋白.
- 为了研究双域 (FOLD) 蛋白在真菌-植物相互作用中的作用.
主要方法:
- 将AM真菌Rhizophagus irregularis的分泌蛋白质结构模型与已知的病原体作用因子进行比较.
- 利用结构相似性搜索来识别保存的效应器家族.
- 分析了AM真菌FOLD基因 (MycFOLDs) 中的基因家族扩张,多样化和积极选择.
- 在植物殖民期间检查了MycFOLD基因表达模式.
主要成果:
- 在AM真菌中鉴定出MycFOLD蛋白质,其结构与病原体FOLD效应器相似.
- 在AM真菌中,MycFOLD基因存在于扩大和多样化的家族中,有积极选择的证据.
- 结构建模表明MycFOLDs具有碳水化合物结合动机.
- 在宿主殖民期间,MycFOLD基因表现出差异性表达,MycFOLD-17在植物树中积累.
结论:
- MycFOLD蛋白广泛分布在与植物无关的殖民真菌中,这表明其功能得到了保护.
- 诱导式表达,序列多样化和在树中的局部化表明MycFOLDs在共生和致病植物殖民中起作用.
- 折叠蛋白代表了参与植物相互作用的保存类真菌效应物.
关键词:
医生们可以使用"Medicago truncatula".花杂交植物 (Petunia hybrida) 是一种植物.阿尔法折叠是什么意思阿尔法折叠这种类型的状菌根真菌是状菌根真菌.效应器 效应器 是一个效应器.在现场混合化.结构模型预测 结构模型预测这种共生是共生.更多相关视频
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