在贝塞特病中,分析口腔和生殖器粘膜表面的微生物组
William Ogunkolade1, Amal A Senusi1, Pareen Desai2
1Centre for Oral Immunobiology and Regenerative Medicine (COIRM), Barts and the London School of Medicine and Dentistry, Queen Mary University of London, UK.
Clinical immunology (Orlando, Fla.)
|May 31, 2023
概括
贝塞特病 (BD) 涉及口腔和生殖器. 这项研究发现了口腔和生殖器微生物组的显著差异,特定的微生物表明活跃的疾病. 像菌素这样的药物也会影响口腔微生物的丰富性.
科学领域:
- 微生物组研究的研究.
- 免疫学 免疫学 免疫学
- 泌尿生殖系统医学
背景情况:
- 贝塞特病 (BD) 经常表现为口腔和/或生殖器,显著影响患者的治疗结果.
- 据认为,BD的病因发生涉及口腔和生殖器微生物组的失调,以及免疫系统的失调.
- 了解这些微生物转变对于识别疾病驱动因素和潜在的治疗点至关重要.
研究的目的:
- 为了研究贝塞特病 (BD) 患者的口腔和生殖器微生物组概况.
- 为了将微生物组组成与疾病严重程度,临床表现和药物使用相关联.
- 为了识别与活性BD和相关的微生物特征.
主要方法:
- 使用高通量16S rRNA测序分析了152名BD患者的口腔和生殖器微生物组.
- 测序针对V1/V2和V3/V4超变区,以捕获全面的微生物特征.
- 微生物组数据与疾病严重程度,工作和社会适应量表 (WSAS) 评分以及药物,包括菌素,相比进行了分析.
主要成果:
- 与口腔样本相比,生殖器样本中的α和β多样性明显较低.
- 在口腔和生殖器部位的部中发现了特定的微生物群落,包括Escherichia-Shigella,Lachnospiraceae,Saccharimonadales和Coriobacteriales.
- 药物,特别是素,显著改变了口腔微生物的丰富性,而某些微生物,如 Streptococcus, Veillonella, Gardnerella 和 Lactobacillus 与活跃的疾病有关.
结论:
- 口腔和生殖器微生物组的组成在贝塞特病 (BD) 患者中显著不同.
- 特定的微生物特征,包括Streptococcus,Veillonella,Gardnerella和Lactobacillus,可以作为活性BD的早期指标.
- 需要进一步研究微生物组,免疫反应和BD中的疾病活性之间的相互作用.
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